![]() However, it is a goal that is difficult to achieve using conventional approaches such as analysis of immune sera by enzyme-linked immunosorbent assay (ELISA). Consequently, the identification of conserved and functionally important antibody epitopes in key antigens is a major goal of malaria immunology research. The extensive inter-clonal polymorphism and intra-clonal variation of key parasite antigens appear to be important reasons. However, acquisition of protection following natural exposure takes years to develop, and complete protection is rarely if ever achieved (reviewed by Hviid, 2005). falciparum malaria following natural exposure is mediated mainly by IgG antibodies with specificity for the asexual blood stages of the infection ( Cohen et al., 1961 Sabchareon et al., 1991). This parasite alone was responsible for an estimated 241 million disease episodes and 627,000 deaths in 2020, mainly in sub-Saharan Africa ( WHO, 2021).Īcquired immunity to P. At least five species of these parasites can cause disease in humans, but by far the most serious is Plasmodium falciparum. ![]() Malaria is a serious infectious disease caused by mosquito-transmitted protozoan parasites of the genus Plasmodium. The assay is adaptable to the analysis of other polymorphic antigens, rendering it a powerful tool in studies of immunity to malaria and many other diseases. Using VAR2CSA-type PfEMP1-a notoriously polymorphic antigen involved in the pathogenesis of placental malaria-as a model, we demonstrate the robustness of the assay and its applicability to analysis of true cross-reactivity of monoclonal VAR2CSA-specific antibodies in naturally exposed individuals. In this study, we present a further enhancement of this assay that makes direct analysis of monoclonal antibody-level cross-reactivity with allelic variants feasible. ELISpot is an assay that enables that, and a recently developed multiplexed variant of ELISpot (FluoroSpot) facilitates simultaneous assessment of B-cell/antibody reactivity to several different antigens. However, serological analysis often does not allow the distinction between true cross-reactivity (one antibody recognizing multiple antigen variants) and apparent cross-reactivity (presence of multiple variant-specific antibodies), as it requires analysis at the single B-cell/monoclonal antibody level. The issue of antibody cross-reactivity is of central importance in immunology, and not least in protective immunity to Plasmodium falciparum malaria, where key antigens show substantial allelic variation (polymorphism).
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The film comes from the 87eleven Entertainment, a production company founded by John Wick franchise director and former stuntman Chad Stahelski with the express purpose of making … well, more John Wick-esque stuff (“The goal will be to generate high octane fare in the action genre, and the hope will be to hatch something that comes out of nowhere at a modest budget and establishes itself as the stylized franchise,” is how Deadline described the company when it formed). The John Wick comparison is earned, or perhaps created, thanks to the combination of forces that worked behind the scenes to make Day Shift happen. Thus Bud finds himself saddled with an anxious by-the-book desk jockey named Seth (Dave Franco) as he scrambles to pull enough fangs to sell before his deadline without breaking any union rules along the way. He calls up his old friend Big John (Snoop Dogg, in a cowboy hat) to give him a recommendation, and the union agrees to give Bud a second chance - provided he has a union rep accompany him on his hunts. Bud needs to make ten grand in seven days or his ex-wife is going to pack up his daughter and move her to Florida, and to do so Bud needs to… be reinstated to the international hunter union that pays the highest price for vampire fangs. Jamie Foxx stars as the improbably named Bud Jablonski, a down-on-his-luck vampire hunter who masquerades as a pool cleaner in sunny Southern California. Welcome to John Wick but with vampires! Welcome to Day Shift! Meanwhile, there’s a whole realm of mediocrity, of fun Friday night escapism, of movies that bite off more than they can chew, that Netflix could easily corner the market on.Īll of which is a very long preamble to say that Netflix has finally found its mediocre sweet spot - its Johnny Mnemonic - and it’s actually a riff on a whole other Keanu Reeves joint. The big stuff feels wasted, with lackluster marketing failing to uplift the properties ( Glass Onion, the first Knives Out sequel, premieres at TIFF next month! It comes out later this year! We’ve seen like 3 photos from the movie so far! WHAT IS HAPPENING?!), while the small stuff is filler and treated as such. Netflix spends hundreds of millions of dollars on securing franchises that should be theatrical tentpoles, like Snyder’s Army of the Dead and the upcoming Knives Out sequels, and then they’ll dump out their loose pocket change on VOD-equivalent fare with no recognizable talent save for a single notable cameo to cut into the trailer (yeah I see you, Megan Fox in Night Teeth). Streaming platforms to a certain extent have helped keep the mid-budget film alive (Hulu’s recent success Palm Springs and even Prey is proof), and while Netflix has been known to occasionally outbid their competitors on an award-winner like The Lost Daughter, it usually sets its sights higher - or much, much lower. Well, logic holds that they should be on Netflix, right? It killed Blockbuster, after all - it should be its destiny to take its place. What I’m saying is: Where are the well-intentioned, fitfully entertaining disasters? Where are the Johnny Mnemonics today? They’d open in third or fifth place at the box office, fade quickly, and then get a bump when the VHS or DVD landed at the rental store, back in the days before Netflix killed Blockbuster. What we don’t often bemoan is the loss of, well, crap - the mediocre movies with big ideas, bankable names, and barely enough talent scraping by behind to camera to keep things afloat. When we talk about the diminished output of mid-budget movies, the conversation is often around cozy dramas or rom-coms or tense psychological thrillers - quality projects that lack the sort of dazzling technical wizardry we’ve been duped into thinking is necessary to warrant a night at the theater. ![]() It’s a cash grab ouroboros of studios devoting resources to sure-bet IP with endless merchandising potential, and theaters devoting screens to whatever lands the most butts in seats and keeps the lights on. The ecosystem of film has shifted a great deal in the past decade or so, with superhero blockbusters crowding out smaller movies at the cineplex (and that’s when going to the movies is even an option, COVID permitting). They can be setup to open and close automatically depending on the weather conditions outside, so you don’t have to worry about adjusting them regularly. They can be set to a certain height and width so that intruders have a hard-time discovering your home.Įnergy efficiency – Roller shades are a great way to keep your home cool in summer and warm in winter. Security – Roller blinds are a great way to ensure your home is safe and secure. You can select a fabric that best suits your preferences. For example, if you want to open or close a roller shade to let fresh air into the room or keep out a chill, you can do so quickly and easily.Ĭustomization – Roller shades allow you to customize your windows. 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These blinds take the place of window blinds, although it is possible to motorize existing blinds. ![]() The benefits of using motorized blinds in your home go well beyond convenience - they can help you save money on energy bills. Motorized roller shades are elements of home automation - a recent trend that more homeowners are starting to adopt. We installed one shower next to the back door. Just because we didn’t install a shower in the bathroom, it doesn’t mean we don’t have one. Showers for campervans come in all shapes and sizes. We still have the bathroom but it’s only used for the toilet and to store our toiletries and towels. So we decided to give the wet room a miss. We hardly ever used the onboard shower and used the room more for hanging coats and our laundry basket than anything else. Part way through the build, we thought more about how we used the bathroom in Mowgli. A million times better than the chemical loo we had. It composts down to nothing and with no smells. We empty the pee pot regularly and the poop pot every 5 or 6 weeks with living in the van full-time. So boys need to sit down to pee and girls need to learn to never pee and poop at the same time. ![]() There’s a knack to using a composting toilet and for greatest effectiveness, the manufacturer recommends keeping wets and solids separate. Having only used it for a couple of weeks, we’re already impressed! It’s not cheap to buy but the composting material is much cheaper than chemicals and better for the environment. So we’ve upgraded from a chemical loo to a campervan composting toilet. Not convinced? Take look here to see how to empty a portapotti! We had a chemical toilet in Mowgli and it was a bloomin’ horrible job to empty it.Īngela would never do it, the chemicals were expensive and sometimes it was hard to find a place to empty it. One of our priorities was to have a toilet on board so we planned to install a bathroom. Sprinter Van Wet Room (which is actually dry) The cost is similar to the cost of a wooden frame but it’s about a third of the weight. ![]() We built the framework from box aluminium. Oh, and we added an extra fuel tank too.Įssential reading before you modify your overland vehicle Sprinter Camper Van Layout & Framework Aside from putting off road tyres on her and engineering a raised air intake, there was no need to make any changes. We’ve kept the van pretty much unmodified. On our test runs up to Scotland and Northumberland, we averaged of 20.1 mpg or about 14 litres/100km. She has a long wheel base and high roof so we can stand up inside without bumping our heads and have space for a bathroom. 4×4 Camper Van – The Base Vehicleīaloo is a Mercedes Sprinter 411 cdi. We spent several month working out what customization options we wanted to install. There were a few rust spots but even then we could see the potential. She wasn’t a cargo van but a fully windowed mini bus. The Mercedes factory fitted 4×4, high and low gear box and diff locks were perfect and she only had 47k miles on the clock. When we first spotted Baloo, she looked a little tired and unloved.
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I've been researching Bed Covers for a long time before i pulled the trigger. 85 + eibachs and Toytec tps for 3'' front lift, wheelers progressive aal w/ overload compustar 2-way remote start Antennex shorty antenna, pop n lock tailgate lock with programmable key, seat covers, Toyota's crappy all-weather floor mats (should have got Husky's or Weathertechs), TRD skid plate, led light pods mounted in front bumper valence w/ Eyourlife wireless harness and remote, led bed lights, light pods mounted in bed, Devil Horns emblem from Diaz Fabrications, rear facing pod lights in bed, under seat lights,Ĭlick to expand.Hey man, wanted some advice since you got the undercover flex 5'' top plate spacer, OME sport front shocks, bilsten 5100s rear, 2'' aal, locking gas cap, LR ucas, Viper 2-way remote start, afe drop in filter, Rocky locking bike mounts, gear chest, scangauge, pop n lock tailgate lockĢ013: most things transferred over from my 07, 5100s. The Undercover Flex features a textured glossy finish on its exterior panels.Joined: Member: #7932 Messages: 2,937 Gender: Male First Name: Matt College Station, TX Vehicle: 2013 Trd Sport DC, 07 DC sr5, 03 Prerunner 2003: StockĢ007: Avid Offroad sliders, 30% front tint, bed mat, Truxedo low-pro qt tonneu cover, aero turbine 2525 muffler w/ 8'' tip, AR25 Resonator, 16x8 Dick Cepek DC-1 w/ 265/75 Goodyear Wrangler Duratracs, 2010 headlights, ome 884 coils with.Click to View Undercover Folding Tonneau Cover Comparison Guide (PDF).Your Undercover Flex Tonneau Cover is crafted right here in the USA and backed by a 3-year warranty.Simple clamp-on system allows for easy, no-drill installation and removal.Fully compatible with under-the-rail bedliners, spray-in bedliners, snug-fitting bed rails and tailgate caps.Hard top surface supports up to 300 lbs of evenly distributed weight.Flush-fit design can’t be opened when the tailgate is locked, providing added security.Integrated drain tubes whisk any accumulating water away from your carg.Hinges and panels won’t crack, warp, fade, rust or corrode in extreme weather.Panels are connected with a seamless, leak-proof rubber seal to lock out moisture.Easy-to-use slam latches allow you to open the cover from either side of the truck.Special prop rods let you flip this truck bed cover completely open and secure in place.Three secure driving positions allow for more hauling versatility and 100% access.Advanced fiberglass panels with reinforced polymer material produce a subtle textured finish.Built tough from fiberglass-reinforced polymer (FRP) and an aluminum top skin panel to keep weather and would-be thieves out.Flex your truck’s cargo-covering muscle with the Undercover Flex Tonneau Cover.Angry Elephant for Honda Ridgeline 2017+ The Undercover Flex hard tonneau folding system is made from fibreglass reinforced polymer that is more resistant than aluminum and provides a low-profile flush design that’s easy to maintain.Malone Auto Racks, Trailers, & Accesories.RetraxPRO XR + Yakima HD T-Slot Tall Rack Package.RetraxPRO XR + Yakima T-Slot Short Rack Package.RetraxONE XR + Yakima T-Slot Short Rack Package.Revolver X4s + Honda Ridgeline Ladder Rack P3000 2017+ (Silver/Black).Revolver X2 + Honda Ridgeline Ladder Rack P3000 2017+ (Silver/Black).RetraxPRO XR + Angry Elephant Rack System.RetraxONE XR + Angry Elephant Rack System.Truxedo Lo Pro + Angry Elephant Rack System.Revolver X2 + Angry Elephant Rack System.Thule Xsporter Pro Rack System (Silver).Yakima T-Slot Pkg Tall Rack for ReTrax XR.Yakima T-Slot Pkg Short Rack for ReTrax XR. ![]() ![]() This stamp set measures approximately 6 x 8 inches and coordinates beautifully with the Boo Crew die set (sssd112176c). 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Share on Facebook Share on Twitter Pin it Simon Says Stamp clear stamps are high quality photopolymer and made in the USA. This stamp set consists of two separate stamps. Simon Says Clear Stamps POPPY FIELD sss202607c 24.99 designer pick. The stamps are designed to work seamlessly with a variety of ink pads, giving you the freedom to experiment with different colors and styles. 24.99 Simon Says Clear Stamps WILDFLOWERS sss201998 Simon Says Stamp clear stamps are high quality photopolymer and made in the USA. ![]()
Hex-Logic engineers rounded the profile of the pad for improved flex into curves and contours and smoother polishing performance. I don't really see the usefullness of a compressor in electronic/modular.Hex-Logic pads are engineered with the finest and most durable detailing foams, hook and loop interface, and V-cut channels to increase airflow and cooling characteristics. 3630 for example.īut maybe I am an outlier. Otherwise a lot of medium to crap quality compressors exist in the analog domain. When I am working in the analog domain, I want to use a DBX160, or something with tubes from the Summit or UA stables. ![]() Even the stock Logic Pro X compressor is very good. But if I am doing compression, I have all these wonderful DAW compressors to choose from. They have this thing where they can transparently control the signal dynamics without adding or removing harmonics. But I just don't really see spending money on a compressor unless it's an all analog, character compressor. The multiband compressor may very well be good as fuck. Since I'm sick of using stompbox reverbs and rack mount reverbs are awkward to use in a modular setup. i find it a lot more powerful and clean to just change the levels of the individual parts in a mix. ![]() but really, why the hell would i wanna run my shit through multi-band compression any way?! it messes with phase response even at neutral settings and has the tendency to completely tear a well constructed mix apart. there's no way you could convince me that the kilpatrick box is higher quality then the native plugs that are available today. I can't imagine bringing that kilpatrick multipress with me any where when i could just as easily bring a laptop and run some DMG plug ins. i like the elektron AH cus it's, well, analog, it has legit distortion and saturation that cannot be copied with a piece of software. i'd rather just bring a real distortion pedal, or a real guitar amp with me. that pedal strikes me as another attempt to stick some plug ins in a tabletop/stomp box. ![]() i dont have the hang ups that others have regarding using a computer to make music (actually i think it's by far the most important tool in a modern setup). If i need a bunch of digital fx for a live set i will simply bring a laptop. Digital, sure, but it's like $60, and 32-bit DSP?! I'm yet to see another company to pull something like that off, def have a look at some of their options (Loop Core Deluxe, Time Force) etc. All the usual emulations there as well: from TS to RAT to Muff, - so a good tone-shaper overall. i would like to see more of these kind of portable but high quality saturation/distortion/clipping boxes out there.Ĭheck out nuX Drive Force: dual-channel stompbox with 32-bit digital signal processing, I think it has EQ and cabinet emulations + noise gate per channel (!), so you can have 2 differently colored output signals that you can mix & match. Supernice mode is the only way I can run them.ĭogoftears wrote:something like the elektron analog heat is a more interesting product to me. They distort bass too much in regular mode. I have never been impressed with the RNC even though I have 2. There is also the ART PRO VLA II which is a leveling amplifier if you change the tubes to Mullards or NOS GE you can get some very good sounds out of these units. Chameleon Labs 7720 is an amazing stereo compressor for buss compression and it is very affordable and has some nice features like a HPF on the detector. And of course, there's the 3930 that everyone love-hates. ![]() DBX also has several models like the 166 that are cheap. Symetrix is a favorite of mine for used gear, like the 525. While you are right if you are talking about a Distressor or some other very high-end compressor, there are lots of good compressors available at reasonable price points in 19" format. Some of them are enormous and mono and you need to buy them in a properly balanced pair if you want to use them on a stereo buss. Studio rackmount compressors are the price of a car. Look at the prices for compressors, the only thing halfway cheap is the Really Nice Compressor. ![]() Trademarks are the distinctive names, words, logos, designs and symbols used to distinguish a product of a particular manufacturer or source. These include copyright in Nintendo's video games themselves, computer software, game visual display, game music, game characters, product packaging, game manuals and labels, hardware chip microcode, artwork and publications.Ĭopyright arises automatically in Australia. There are several different types of copyright which are associated with Nintendo's products. CopyrightĬopyright is the exclusive right granted to an author of a literary, musical, audiovisual or artistic work, to reproduce and distribute that work or to make it available online. Nintendo Switch Intellectual Property NoticesĬlick here for information on intellectual property notices about the Nintendo Switch family. These include copyright, trademarks, and patents, designs and circuit layouts. Nintendo owns intellectual property rights in its products. We appreciate your interest in Nintendo brands and products. Nintendo treats very seriously any infringement of its rights. Nintendo does not give legal advice, please act with care. ![]() If you are uncertain about whether your planned activities require Nintendo's consent you should obtain your own legal advice. The fact that we do not respond does not imply permission for you to use any of Nintendo's intellectual property. Because we receive so many requests for use we cannot respond to them all individually. You are not entitled to use any of this Nintendo property without our specific permission. Nintendo has over 300 trademarks registered in Australia. This includes the name Nintendo, logos, characters, product names, games, graphics and website and marketing content. The Nintendo brand and Nintendo products are protected by intellectual property laws. Nintendo will continue to pursue the distribution of game copying devices on a global scale. In 2009 alone, working with law enforcement agencies, Nintendo has pursued actions against over 80 factories in China producing the unlawful devices. As part of its battle against piracy, Nintendo is also working with Chinese enforcement authorities to pursue factories in China responsible for the manufacture of the infringing devices. Nintendo has had assistance from law enforcement authorities in Australia, Belgium, Denmark, France, Germany, Hong Kong, Italy, Japan, Korea, the Netherlands, New Zealand, Singapore, Spain, Taiwan, United Kingdom and United States. Since 2008, Nintendo has supported over 600 actions in 16 countries, confiscating over a half million DS game copiers. Nintendo has also been active in its efforts to stop the illegal distribution of game copying devices. For over 20 years, Nintendo has undertaken an aggressive worldwide campaign to stop the production and distribution of pirated video game products. Unfortunately, Nintendo and its over 100 independent publishers and developers, lose hundreds of millions of dollars per year to international pirates and counterfeiters.Ĭounterfeiting of Nintendo's video game products is a serious problem. ![]() It costs millions of dollars to develop, manufacture, market and distribute a single Nintendo video game. The creation of a single video game often takes several years of work for teams of game developers, artists, animators, musicians, motion capture artists and many others. ![]() Nintendo has become a household word around the globe, and its video games have become a multi-billion dollar industry. This section of our website is designed to answer questions you may have about our intellectual property rights and Nintendo's response to infringement of those rights. |
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